-Custom Sevices of Protein Expression by the pin-point labeling- [Principle]   Labeling position of a protein is decided by the UAG amber codon which is one of the stop codons or the CGGG codon (four-base codon).   An unnatural aminoacyl-tRNA recognizes the UAG amber codon or the CGGG codon during translation. Consequently, the unnatural amino acid is incorporated at a defined position of a protein. Two types of unnatural amino acids are able to incorporate into a protein using two types of tRNAs recognizing codons of the amber and the CGGG at the same time, and the dual labeled protein can be used as measurements such as the fluorescence resonance energy transfer (FRET) and other applications.   Please refer to the following linkage page about details For amber codon method >> principle of CloverDirect TM For CGGG codon (four-base codon) method >> principle of In vitro Pin-point Labeling Kit 543 Overview of the pin-point labeling technology using unnatural aminoacyl-tRNA [About Unnatural aminoacyl-tRNA]   You can get synthetic aminoacylated tRNAs that carries unnatural amino acids of your interest. We take on synthesis of your original tRNA. Using CloverDirect TM reagents, it is possible to express proteins which are labeled with fluorescent dyes (HiLyte Fluor 488, TAMRA, ATTO633) or BIotin. Please refer to the following linkage page about details.       Custom Synthesis of Unnatural Aminoacyl-tRNA (Examples of Unnatural amino acids) Fluorescent labeling         Amino acid labeled with Fluorescein, Rhodamine, etc. Biotin labeling         Amino acid labeled with Biotin、Biotin-X, etc. Cross linker         p-Benzolylphenylalanine         p-Acetylphenylalanine Mimic post-translational modification         (Epsilon)-Methyl-Lysine         (Epsilon)-Dimethyl-Lysine        (Epsilon)-Acetyl-Lysine Peglylated amino acid [Questions about Products] >>Online question Back to HOME Back to Custom Service Groups Back to "Pin-point Protein Labeling" page