We can synthesize a tRNA charged with an unnatural amino acid (fluorescent-labeled or functional amino acid, etc.) suitable for your researches. So, you can get pin-point labeled proteins with your original amino acids.
  We can comprehensively perform from construction of DNA templates for in vitro translation to production/purification of labeled proteins using pin-point labeling technology. See details in the following link.

                   Custom Services of Protein Expression by the Pin-point Labeling

1. INQUERY :  Contact us by E-mail

2. CONSULTATION :  We reply to you. And we ask some questions about tRNAs charged with unnatural amino acids of what you need.

3. DESIGN PROPOSITION :  We propose the designs based on your purpose, and we also indicate the prices of tRNA synthesize services.

4. CONFIRMATION :  Please confirm these designs and prices. If you tell your acceptance of our proposition to us, we would send order sheet (pdf file) to you by E-mail. We start syntheses of the tRNAs as soon as arriving order sheet with your signature

5. DELIVERY OF PRODUCTS :  We deliver the products and the reports of syntheses after completion.

We have revealed that synthesized aminoacyl-tRNA containing base structure of p-amino-phenylalanine is good substrate for this system. So we recommend that the design of unnatural amino acids are based on p- aminophenylalanine structure and are bond to fluorescent or functional groups at p-NH₂ position of the aminophenylalanine.
We can also synthesize tRNAs with natural or unnatural amino acids except for derivatives containing the amino-phenylalanine structure. If you have other ideas about tRNA design, feel free to contact us.

Incorporation of an unnatural amino acid in translation depends heavily on its structure. Some unnatural amino acids are difficult to incorporate into proteins or are restricted the incorporation to particular positions in protein.
We can’t warrant your achievement of producing modified protein for all products of this service. But, we can almost predict success rate of the protein production by consideration of a designed amino acid structure and our current researches.

The molar mass of the final product is not warranted, because chemical character of the unnatural amino acid frequently influences on the final yield of product tRNA.
 We can perform bulk scale of the tRNA production.


[DELIVERY DATE]

Over 4 weeks from order in Japan

The required period to delivery depends on how many steps are needed in the tRNA synthesis. We will tell you the required period at the same time of design proposition.


[PRICE]

(EXAMPLE) Fluorescein charged tRNA : ¥164,000- / 8 µmol (=1mL translation Reaction)

  Price of fluorescein dye excepted.


[EXPIRATION DATE]

Freeze dried tRNA^*1: 12 months^*2, stored at -80°C
Reconstituted tRNA^*1: 3 months^*2, stored at -80°C

*1. Same as CloverDirect^TM reagents, the product of this service is delivered as freeze dried tRNA with tRNA buffer for reconstitution.
*2. These expiration dates are not warranted, because the tRNA stability is fairly influenced by stability of unnatural amino acid charged.


[CONFIDENTIAL DISCLOSURE AGREEMENT (CDA)]

If you hope, the CDA concerned with this service will be concluded.


[References]

1) FRET analysis of protein conformational change through position-specific incorporation of fluorescent amino acids
Daisuke Kajihara, Ryoji Abe, Issei Iijima, Chie Komiyama, Masahiko Sisido, Takahiro Hohsaka
Nature Methods, 3, 923-929 (2006).

2) Position-specific incorporation of biotinylated non-natural amino acids into a protein in a cell-free translation system
Takayoshi Watanabe, Norihito Muranaka, Issei Iijima, and Takahiro Hohsaka
Biochem. Biophys. Res. Commun., 361, 794-799 (2007)


[Questions about Products]

>>Online question


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