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| Products |
Storage
conditions |
Cat. No. |
Size / 1 kit |
Price(\) |
| CloverDirect TM HiLyte
Fluor 488 |
-80°C |
CLD01 |
300 µL
translation |
\28,500- |
| CLD05 |
5 X
300 µL translation |
\128,500- |
| CloverDirect TM
TAMRA |
-80°C |
CLD02 |
300 µL
translation |
\28,500- |
| CLD06 |
5 X
300 µL translation |
\128,500- |
| CloverDirect TM ATTO
633 |
-80°C |
CLD03 |
300 µL
translation |
\40,000- |
| CLD07 |
5 X
300 µL translation |
\180,000- |
| CloverDirect TM
Biotin |
-80°C |
CLD04 |
300 µL
translation |
\20,000- |
| CLD05 |
5 X
300 µL translation |
\90,000- | | |
|
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[Description]
CloverDirect TM tRNA Reagent for
Site-Directed Protein Labeling allows the pin-point labeling by
fluorescence dyes or biotin at a defined position of protein in
E.coli cell-free translation.
The labeled
protein will be produced by just adding CloverDirect
TM reagent and the DNA template which is replaced at a
particular position with amber stop codon (UAG) to in vitro
translation system.
[Features]
Labeling position is decided by an amber codon which is the one of
stop codons.
 Pin-point labeling allows selection of a
labeling position which hardly interferes with protein structure and
function in comparison to other methods (e.g., chemical labeling and
GFP fusion production).
Labeled aminoacyl-tRNAs are able to choose
from three fluorescent dyes (HiLyte Fluor 488, TAMRA, ATTO633) for
488 nm, 543 nm and 633 nm excitation lasers, respectively.
Pin-point fluorescence labeled proteins greatly
facilitate analyses in single molecule fluorescence analysis,
fluorescence energy transfer (FRET), cell imaging, and other
applications.
Pin-point biotin labeled proteins can be used for
efficient immobilization by biotin-avidin
interaction.
[Product
Contents]
 Labeled
aminoacyl-tRNA, freeze dried (Note 1) X
1
tRNA reconstitution
buffer X
1
These products do not include cell extract for in
vitro translation. Prepare them separately by
user.
E.coli extract (e.g., Roche Diagnostics, RTS 100
E.coli HY kit) is recommended.
(Note 1) The following labeled aminoacyl-tRNA
can be supplied. |
| Labeled aminoacyl-tRNA |
Dye |
Excitation (nm) |
Emission (nm) |
| CloverDirect TM HiLyte Fluor
488 |
HiLyte
Fluor 488 |
497 |
525 |
| CloverDirect TM TAMRA |
TAMRA |
546 |
575 |
| CloverDirect TM ATTO
633 |
ATTO
633 |
629 |
657 |
| CloverDirect TM Biotin |
Biotin |
- |
- | |
[References]
1) FRET analysis of protein conformational
change through position-specific incorporation of fluorescent amino
acids
Daisuke Kajihara, Ryoji Abe, Issei Iijima, Chie Komiyama,
Masahiko Sisido, Takahiro Hohsaka
Nature Methods, 3,
923-929 (2006).
2) Position-specific incorporation of
biotinylated non-natural amino acids into a protein in a cell-free
translation system
Takayoshi Watanabe, Norihito Muranaka, Issei
Iijima, and Takahiro Hohsaka
Biochem. Biophys. Res.
Commun., 361, 794-799 (2007)
[Questions about Products]
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question
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