Custom service of the protein expression (E. coli)
<<OUTLINE>>
·The target protein is expressed by E. coli expression system.
·We can construct the expression vectors even when you don't have
your desired gene.
·We are able to optimize the expression conditions, to culture by large-scale and to purify the target protein.
<<Contents of Service>>
| Construction of the Expression Vector |
The expression vector is constructed after receiving the cDNA from customers.
If you don't have the cDNA, we can obtain the cDNA by our custom gene synthesis
service. |
 |
| Trial Expression |
Productivity and solubility of the target protein are confirmed by the
small scale. |
|
| Optimization |
Conditions of the protein expression are optimized (host, induction, medium,
cultivation time and so on). |
|
| Scale-up |
The scale can be adjusted your desired amount. |
|
| Purification |
The target protein is purified. |
|
Contents of Service |
| Plasmid Construction |
| CS301 |
Plasmid Construction (E. coli) |
| Trial expression |
| CS302 |
Expression (E. coli) |
| Scale-up |
| CS303 |
Expression (E. coli), 100ml |
| CS304 |
Expression (E. coli), 1200ml |
| CS305 |
Expression (E. coli), 4800ml |
| CS306 |
Expression (E. coli), 20L |
| Purification |
| CS307 |
Metal-Affinity column, 1mL (E. coli) |
| CS308 |
Metal-Affinity column, 5mL (E. coli) |
Technical Inquiry
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