·Target proteins are expressed by cell-free translation system (E.coli or wheat germ).

·This translation system is the T7 promoter system.

·It is possible to construct expression vectors when you don't have your desired gene.

·PCR products can be also used as a template in this system.

·We are able to optimize the expression conditions, to produce large scale and to purify the target protein.

·We can express the target protein having disulfide bonds.

Preparation of the Template	The expression template (plasmid or PCR product) is constructed after receiving the cDNA from customers. When you don't have the cDNA, it is possible to obtain the cDNA by our custom gene synthesis service.
Trial Expression	Productivity and solubility of the target protein are confirmed by small scale (50µl).
Optimization	Conditions of the protein expression are optimized (addition of surfactants, chaperone and so on).
Scale-up	The scale can be adjusted to your desired amount.
Purification	Produced protein is purified.

	Contents of Service
Preparation of the template
CS101	Linear template synthesis (cell-free)*
CS102	Plasmid Construction (cell-free)
CS103	Plasmid preparation by large scale
Trial expression
CS104	Expression (cell-free), 50µl
Scale-up
CS105	Expression (cell-free), 1ml
CS106	Expression (cell-free), 10ml
Purification
CS107	Metal-Affinity column, 1mL (cell-free)
CS108	Metal-Affinity column, 5mL (cell-free)

*We synthesize both N- and C- terminally tagged DNA in Linear template synthesis.