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In vitro Pin-point Fluorescence Labeling Kit 543 |
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Pin-point protein labeling by TAMRA for Single Molecule Fluorescence Detection
System MF20 (Olympus Co.,Ltd.) using 543 nm laser.
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Pin-point protein labeling
by expanded four base codon technology!! |
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| Product |
Storage conditions |
Cat. No. |
Size |
Price(¥) |
| In vitro Pin-point Fluorescence Labeling Kit 543 |
2~8°C /
-30~ -15°C |
N2904200 |
2 X 150 µL translation scale |
¥134,400- |
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| Link to distributor's page : OLYMPUS Co. Ltd. |
[Product information]
In vitro Pin-point Fluorescence Labeling Kit 543 is composed
of two units; Labeling unit and Purification unit.
Labeling unit : This unit includes reagents for pin-point TAMRA labeling. Pin-point TAMRA
labeled protein will be produced by using this unit with in vitro E.coli translation system in common use. The fluorescent dye, TAMRA, is suitable
for Single Molecule Fluorescence Detection System MF20 (Olympus Co.,Ltd.)
using 543nm laser.
Purification unit : This unit is possible to purify and concentrate the proteins labeled with
TAMRA .
Production of positive control CAT (Chloramphenicol acetyltransferase):
One mg of pin-point TAMRA labeled CAT will be prepared by this kit at 300
µl reaction. This amount of pin-point TAMRA labeled CAT suffices
to 384 samples for multi-well analysis using Single Molecule Fluorescence
Detection System MF20.
[Features]
Pin-point protein labeling is performed by
expanded four base codon technology.
Pin-point labeling is able to label at only one position. In
minimization of labeling effect on protein activity and structure etc.,
our method has advantages over other methods (e.g., chemical labeling and
GFP fusion).
Pin-point labeled
protein will be useful for Single Molecule Fluorescence Detection System.
[Kit contents]
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| Labeling unit ( store at -30~ -15°C) |
number |
| TAMRA-tRNA (see note 1) |
2 tubes |
| tRNA buffer |
1 tube |
| pROX-FL (100ng/µL) |
1 tube |
| Control Vector CAT (100ng/µL) |
1 tube |
| Purification unit (store at 4~ 8°C) |
number |
| Phosphate/NaCl buffer (8X) |
2 tubes |
| imidazole (4M) |
1 tube |
| Polyoxythylene(23)Lauryl Ether (8X) |
1 tube |
| PBS (8X) |
1 tube |
| His Spin TrapTM Columns (GE Healthcare Science) |
2 tubes |
| ULTRAFREE-0.5 Centrifugal Filter Devices 10k (Millipore) |
2 tubes |
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(note 1) Fluorescent characteristic of TAMRA-tRNA |
| Dye |
Excitation (nm) |
Emission (nm) |
| TAMRA |
546 |
575 |
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[References]
1) FRET analysis of protein conformational change through position-specific incorporation of fluorescent amino acids
Daisuke Kajihara, Ryoji Abe, Issei Iijima, Chie Komiyama, Masahiko Sisido, Takahiro Hohsaka
Nature Methods, 3, 923-929 (2006).
[Questions about Products]
>>Online question
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